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MPO ELISA EIA髓過(guò)氧化物酶試劑盒背景介紹：Mercodia MPO ELISA provides a method for the quantitative determination of human MPO in EDTA-plasma.

MPO ELISA EIA髓過(guò)氧化物酶試劑盒Summary and explanation of the test Myeloperoxidase (MPO), an iron containing glycoprotein, is a covalently bound tetrameric complex with a molecular weight of 150 kDa. It is composed of two glycosylated alfa chains of MW 59-64 kDa and two unglycosylated beta chains of MW 14 kDa. MPO is found in abundance in the primary azurophilic granules of neutrophils and is present in monocytes. ?In response to microbial invasion, MPO is released from the cytoplasmic granules of neutrophils into the phagosome and extracellular space, catalysing the conversion of hydrogen peroxide and chloride ions (Cl- ) into hypochlorous acid, a potent oxidizing agent. ?Myeloperoxidase traditionally is used as a marker of airway inflammation caused by asthma or environmental irritants. It is also believed that MPO participates in different stages of atherogenesis and has a potential role in the promotion of atherosclerosis. Association between elevated MPO levels in serum and cardiovascular disease (CAD) supports an important role for MPO as an inflammatory marker in CAD, making it possible to identify patients at risk for cardiac events in the absence of mycardial necrosis.

MPO ELISA EIA髓過(guò)氧化物酶試劑盒Principle of the procedure Mercodia MPO ELISA is a solid phase two-site enzyme immunoassay. It is based on the sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the MPO-molecule. During incubation, MPO in the sample react with anti-MPO antibodies bound to microtitration wells. After washing, peroxidase conjugated anti-MPO antibodies are added and after the second incubation and a simple washing step that removes unbounded enzyme labeled antibody, the bound conjugate is detected by reaction with 3,3’,5,5’-tetramethylbenzidine (TMB). The reaction is stopped by adding acid to give a colorimetric endpoint that is read spectrophotometrically。